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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/05/2017 |
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Data da última atualização: |
29/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z. |
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Afiliação: |
Carolina Cerqueira Sarmento Olivares; Joanna Maria Gonçalves de Souza-Fabjan; JEFERSON FERREIRA DA FONSECA, CNPC; Mario Felipe Alvarez Balaro; Vicente José de Figueirêdo Freitas; Rodrigo Vasconcelos de Oliveira; Felipe Zandonadi Brandão. |
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Título: |
Comparison of different sperm selection techniques in ram frozen-thawed sperm. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 45, p. 1431, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Resumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of interval incubation. However, the latter three techniques presented more (P < 0.05) reacted cells. Swim-up recovered a higher (P < 0.05) number of intact cells (32.1 ± 6.4%), and Percoll, mini-Percoll and control group were similar (P > 0.05). Discussion: The present study evaluated the sperm cell and its preparation for receiving the oocyte under optimal conditions. evaluate the sperm cell and its preparation for receiving the oocyte under optimal conditions. Although the swim-up technique promoted higher rates for some of the sperm parameters evaluated, Percoll protocols are the most widely used procedures for selection during the sperm preparation in many species, possibly because of its greater speed, practical method and convenience compared to the swim-up technique. Percoll and mini-Percoll recovered approximately 10 times more cells than swim-up, which is an important feature to be considered during sperm preparation for in vitro fertilization (IVF), being possible to use only one semen straw. The high capacitated and acrosome reaction rates observed after the treatments in the current study, are probably reinforced by changes in sperm cells caused by the cryopreservation process. In order to strength the evidence that frozen-thawed sperm, even after selection, is sensible and reactive to capacitation-like events, we demonstrated the capacitated and acrosome-reacted cell values immediately after the selection treatments behaved differently than when authors used ram fresh sperm. Possibly, this capacitation-like changes observed in frozen-thawed sperm occurs regardless of the selection treatment used. Analyzing the motility parameters immediately after the selection, all treatments maintained or increased the rates compared to the control group. The swim-up, mini-Percoll and Percoll did not differ in any parameters. Given that Percoll and mini-Percoll did not show differences in relation to swim-up for motility parameters, such techniques can be used to replace the latter, obtaining similar sperm samples with good quality. However, swim-up technique involves a procedure that recovers a clean fraction without debris and other types of cells, with high rate of mobile sperm with excellent quality, reason why it can justify the higher recovery of intact spermatozoa after the technique. MenosResumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of inte... Mostrar Tudo |
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Palavras-Chave: |
CASA; CTC; Plasma membrane integrity; Protocolo; Reproductive techniques; Sperm preparation. |
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Thesagro: |
Espermatozóide; Ovino; Reprodução animal; Sêmen. |
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Thesaurus Nal: |
Reproduction; Sheep; sperm capacitation; Spermatozoa; Treaties. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160322/1/CNPC-2017-Comparison.pdf
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Marc: |
LEADER 04855naa a2200373 a 4500
001 2070178
005 2017-05-29
008 2017 bl uuuu u00u1 u #d
100 1 $aOLIVARES, C. C. S.
245 $aComparison of different sperm selection techniques in ram frozen-thawed sperm.
260 $c2017
520 $aResumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of interval incubation. However, the latter three techniques presented more (P < 0.05) reacted cells. Swim-up recovered a higher (P < 0.05) number of intact cells (32.1 ± 6.4%), and Percoll, mini-Percoll and control group were similar (P > 0.05). Discussion: The present study evaluated the sperm cell and its preparation for receiving the oocyte under optimal conditions. evaluate the sperm cell and its preparation for receiving the oocyte under optimal conditions. Although the swim-up technique promoted higher rates for some of the sperm parameters evaluated, Percoll protocols are the most widely used procedures for selection during the sperm preparation in many species, possibly because of its greater speed, practical method and convenience compared to the swim-up technique. Percoll and mini-Percoll recovered approximately 10 times more cells than swim-up, which is an important feature to be considered during sperm preparation for in vitro fertilization (IVF), being possible to use only one semen straw. The high capacitated and acrosome reaction rates observed after the treatments in the current study, are probably reinforced by changes in sperm cells caused by the cryopreservation process. In order to strength the evidence that frozen-thawed sperm, even after selection, is sensible and reactive to capacitation-like events, we demonstrated the capacitated and acrosome-reacted cell values immediately after the selection treatments behaved differently than when authors used ram fresh sperm. Possibly, this capacitation-like changes observed in frozen-thawed sperm occurs regardless of the selection treatment used. Analyzing the motility parameters immediately after the selection, all treatments maintained or increased the rates compared to the control group. The swim-up, mini-Percoll and Percoll did not differ in any parameters. Given that Percoll and mini-Percoll did not show differences in relation to swim-up for motility parameters, such techniques can be used to replace the latter, obtaining similar sperm samples with good quality. However, swim-up technique involves a procedure that recovers a clean fraction without debris and other types of cells, with high rate of mobile sperm with excellent quality, reason why it can justify the higher recovery of intact spermatozoa after the technique.
650 $aReproduction
650 $aSheep
650 $asperm capacitation
650 $aSpermatozoa
650 $aTreaties
650 $aEspermatozóide
650 $aOvino
650 $aReprodução animal
650 $aSêmen
653 $aCASA
653 $aCTC
653 $aPlasma membrane integrity
653 $aProtocolo
653 $aReproductive techniques
653 $aSperm preparation
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aFONSECA, J. F. da
700 1 $aBALARO, M. F. A.
700 1 $aFREITAS, V. J. de F.
700 1 $aOLIVEIRA, R. V. de
700 1 $aBRANDÃO, F. Z.
773 $tActa Scientiae Veterinariae$gv. 45, p. 1431, 2017.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 22 | |
| 4. |  | MAIA, A. L. R. e S.; SOUZA-FABJAN, J. M. G. de; RANGEL, P. S. C.; CÔRTES, L. R.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Estrus induction in the non-breeding season is not associated with hydrometra in dairy goats. Research, Society and Development, v. 10, n. 11, e21101119162, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 5. | | TEIXEIRA, T. A.; FONSECA, J. F. da; SOUZA-FABJAN, J. M. G. de; CARVALHEIRA, L. de R.; FERNANDES, D. A. de M.; BRANDÃO, F. Z. Efficiency of different hormonal treatments for estrus synchronization in tropical Santa Inês sheep. Tropical Animal Health and Production, Heidelberg, v. 48, n. 3, p. 545-551, Mar. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
|  |
| 7. | | SOUZA-FABJAN, J. M. G. de; OLIVARES, C. C. S.; VELTRI, A. B. P.; BALARO, M. F. A.; FREITAS, V. J. de F.; FONSECA, J. F. da; BRANDÃO, F. Z. Avaliação da longevidade espermática após o uso de diferentes métodos de seleção de espermatozóides ovinos congelados. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 21., 2015, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2015. p. 199.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | CORTAT, P. R.; DIAS, M. B.; PAULA, C. J. C. de; RANGEL, P. S. C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da. Correlation between estrus onset and ovarian parameters in dairy goats. Ciência Animal Brasileira, v. 23, e-70813E, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z. Comparison of different sperm selection techniques in ram frozen-thawed sperm. Acta Scientiae Veterinariae, v. 45, p. 1431, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z. Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin. Acta Scientiae Veterinariae, v. 47, Pub. 1707, p. 1-8, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. Animal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 12. | | BRAGANÇA, G. M.; ALFRADIQUE, V. A. P.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. de; ARASHIRO, E. K. N.; BALARO, M. F. A.; OLIVEIRA, I. C. de; BRANDÃO, F. Z.; CÔRTES, L. R.; FONSECA, J. F. da. Efeito de diferentes protocolos hormonais para superestimulação ovariana sobre o número e a qualidade de oócitos em ovelhas da raça Santa Inês. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 470, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | VERGANI, G. B.; FONSECA, J. F. da; TREVIZAN, J. T.; PEREIRA, V. S. do A.; GARCIA, A. R.; ESTEVES, S. N.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G. de; OLIVEIRA, M. E. F. Effects of hCG administered 7.5 days after synchronous estrus induction during the non-breading season in Morada Nova ewes. Animal Reproduction, v.17, n.3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | VERGANI, G. B.; FONSECA, J. F. da; TREVIZAN, J. T.; PEREIRA, V. S. do A.; GARCIA, A. R.; ESTEVES, S. N.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G. de; OLIVEIRA, M. E. F. Effects of hCG administered 7.5 days after synchronous estrus induction during the non-breading season in Morada Nova ewes. Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | BRAGANÇA, G. M.; BALARO, M. F. A.; FONSECA, J. F. da; PINTO, P. H. N.; ROSA, R. M. da; RIBEIRO, L. S.; ALMEIDA, M. S.; SOUZA FABJAN, J. M. G. de; GARCIA, A. R.; BRANDÃO, F. Z. Ultrassonografia doppler no diagnóstico precoce da gestação em ovinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, v. 30, Foz do Iguaçu, 2016. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. p. 292. Resumo SBTE 175.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | TAIRA, A. R.; SANTOS, J. D. R.; COSENTINO, I. O.; BRAIR, V. L.; ESPÍRITO SANTO, C. G. do; PINTO, P. H. N.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, J. Z. The use of exogenous progesterone in sheep superovulation protocols influences the percentage of viable embryos. Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos. Animal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDXI expression in in vivo-produced ovine embryos. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECNNOLOGY SOCIETY, 14., 2017, Cabo de Santo Agostinho. Proceedings... Cabo de Santo Agostinho: SBTE, 2017. p. 901.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. Theriogenology, v. 105, p. 150-157, Jan. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDAO, F. Z. L-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression. Theriogenology, v. 105, p. 150-157, jan. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 22 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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